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构建基因表达载体的目的就是使目的基因能够在受体细胞中表达和发挥作用 双子叶植物体受到损伤时，伤口细胞会分泌大量的酚类化合物吸引农杆菌 含有某种生物全部基因的许多DNA片断称为基因组文库 用E.coliDNA连接酶可将Smal限制酶切割时产生的末端连接起来
图1所示的质粒分子经Sma l切割后，含2个游离的磷酸基团 对图中质粒进行改造，插入的Smal酶切位点越多，质粒的热稳定性越高 用图中的质粒和外源DNA构建重组质粒，可使用Smal切割 构建重组质粒时，要使用DNA连接酶。
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other countries do not have fine weather. you cannot experience four seasons in a year in England. the weather in England often changes and is therefore unique. the weather in England never changes.
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